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KMID : 0377519940190010001
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Chung-Ang Journal of Medicine
1994 Volume.19 No. 1 p.1 ~ p.16
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Pathological Study of Selenium Effect on Cellular Proliferation of Preneoplastic Nodules in Murine Hepatocarcinogenesis
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Abstract
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To evaluate the anticarcinogenic effects of selenium(Se) on the hepatic carcinogenesis, analysis of histopathology was conducted and proliferation indeces were monitored during the experiment according to the Solt-Farber method. Sprague Dawley
rats
were
subjected to chemical hepatocarcinogenic procedure by diethylnitrosamine(DEN) followed by 70% partial hepatectomy(PH) and acetylaminofluorene(AAF). The histopathological and immunohistochemical stain for the proliferating cell nuclear
antigen(PCNA)
antibody were carried out, Experimental groups were divided into five group ; D(DEN only), DS(DEN+Se), DA(DEN+AAF) and DAS(DEN+AAF+Se) and control(PH only).
Histopathological analysis revealed small and multiple transformed foci of clear cells in the D and the DS group while large and definite nodules of clear, eosinophil and mixed cellularity were developed in the DA and the DAS group even in the
ealry
stage. Basophilic nodule, adenoma and carcinoma were noted in later stage so called nodule in nodule fashion especially in the DA and the DAS group while they were not detected int he D and the DS group. Proliferating indeces were similar in
nodule
and
surrounding liver cell in the D and the DS group at posthepatectomy 3rd month while the indeces significantly increased in hyperplastic nodules of the DA and the DAS group in the later stage. Transformed foci detected by immunohistochemistry of
glutathione S-transferase revealed significant increased of poistive areas in the DA and the DAS group compared with the D and the DS group.
Proliferating indeces in hyperplastic nodule and GST-P positive foci increased in persistent nodule in the later stage of hepatic carcinogenesis with weak inhibitory effects. Inhibitory action of selenium was slight in this model of murine
hepatocarcinogenesis.
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KEYWORD
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